|
Abstract : |
The chick embryos, after 24 h of incubation were treated in front of the head area with three different doses of tamoxifen (100, 200 or 400 ?g). There were no effects of the tamoxifen on the low dose group while it caused around 16% of the sterility of embryos in other treated groups. Embryos of higher treated groups showed more primordial germ cells (PGCs) remaining in the area of germinal crescent (GC) compared with zero cells in the control group. Also there was a significant reduction in the number of PGCs in the gonad area of the treated group as compared with the control group. So, tamoxifen prevented the migration of PGCs from GC possibly by occupying calcium-binding sites on calmodulin, or by inhibiting the gene expression of the microtubule formation in PGCs and caused the sterilization of the chick embryos. Tamoxifen had less side effects causing only~0.07% embryo abnormalities, thus can be used for producing transgenic chick embryo by PGCs transfer., The chick embryos, after 24 h of incubation were treated in front of the head area with three different doses of tamoxifen (100, 200 or 400 g). There were no effects of the tamoxifen on the low dose group while it caused around 16% of the sterility of embryos in other treated groups. Embryos of higher treated groups showed more primordial germ cells (PGCs) remaining in the area of germinal crescent (GC) compared with zero cells in the control group. Also there was a significant reduction in the number of PGCs in the gonad area of the treated group as compared with the control group. So, tamoxifen prevented the migration of PGCs from GC possibly by occupying calcium-binding sites on calmodulin, or by inhibiting the gene expression of the microtubule formation in PGCs and caused the sterilization of the chick embryos. Tamoxifen had less side effects causing only~0.07% embryo abnormalities, thus can be used for producing transgenic chick embryo by PGCs transfer., |
