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Abstract : |
A protocol for rapid multiplication of strawberry Fragaria x ananasa Duch cv. Chandler has been described. Meristem tips were cultured on MS and Knop medium containing benzylaminopurine (BAP) at 0.5, 1.0, 2.0 and 5.0 mg/l. Various clusters of micro-shoots (300 micro-shoots/culture) were obtained on MS medium with 1.0 mg/l BAP. Knops medium gave best response when enriched with 0.5 mg/l BAP. Complete plantlets were produced by transferring the shoot buds on hormone free MS or Knops medium containing 0.1 mg/l IBA. , A protocol for rapid multiplication of strawberry Fragaria x ananasa Duch cv. Chandler has been described. Meristem tips were cultured on MS and Knop medium containing benzylaminopurine (BAP) at 0.5, 1.0, 2.0 and 5.0 mg/l. Various clusters of micro-shoots (300 micro-shoots/culture) were obtained on MS medium with 1.0 mg/l BAP. Knops medium gave best response when enriched with 0.5 mg/l BAP. Complete plantlets were produced by transferring the shoot buds on hormone free MS or Knops medium containing 0.1 mg/l IBA., |