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Abstract : |
Isolate USM A10 which was identified to be Aspergillus flavus was selected from a screening programme for lipase production via a solid state fermentation system (SSF). The optimization of lipase production by A. flavus USM A10 using rice husks and rubber wood dusts as substrates revealed a maximum production of about 7.0 U g-1 substrate and growth of 0.20 mg glucosamine g-1 substrate. The productivity of lipase after optimization was 700% higher of 1.42 U h-1 mg-1 glucosamine compared to before optimization of 0.2 U h-1 mg-1 glucosamine. The optimized medium and cultural conditions consisted of wood dusts and rice husks of size 2.0 mm at the ratio of 1:1, 90% moisture content and inoculum size of 5x105 spores/g at 28C. The addition of sesame oil at 6% (w/w), maltose 0.15% (w/w) and (NH4) H2PO4 0.05% (w/w) also enhanced the lipase production by the fungus which is about 150% higher than without any supplementation., |
