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Abstract : |
Mantisia spathulata (Zingiberaceae) is a rare and endangered, endemic plant of North-East India. The plant has been rediscovered from Mizoram, India after two decades. Successful recovery of the plant has been achieved through an efficient true-to-type micropropagation protocol. Various concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) were tried for in vitro plantlet production from rhizomatous shoot explants. Optimum shoot and root multiplication was obtained in 8 weeks using rhizomatous shoot explants cultured on Murashige and Skoog medium supplemented with 11.1 أƒإ½أ‚آ¼M BAP and 2.7 أƒإ½أ‚آ¼M NAA. The in vitro plantlets were successfully weaned and transferred to soil with about 90-93% survival rate. So far, more than 1000 plants have been produced successfully and reintroduced into nature for the recovery of this species., |
