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Abstract : |
An attempt was made to study RAPD using 30 isolates of S. enteritidis obtained from chicken samples for the purpose of strain discrimination. The primer used was P1254, (5`CCGCAGCCAA3`) to amplify the genomic fragment of S. enteritidis isolates, which resulted in production of six clones. Six isolates each in clone 1, 2 and 5 and another four isolates each in clone 2, 4 and 6, respectively. In clone 1, having an additional 900 bp amplified products but it was lacking in clones 2 and shared a common 500 bp fragments. Clone 3 had additional amplified fragment at 450 bp. It was lacking in clone 4 and clone 5 had the additional amplified products at 950, 900, 750 and 560 bp but it was lacking in clone 6. The final cluster 6 had three fragments with molecular weight 1550, 1300 and 400 bp, respectively. To sum up, there were 6 distinct clones of S. enteritidis serovars identified based on RAPD profiling., |
