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Abstract : |
Polyclonal antibodies against avian paramyxovirus-1 (APMV-1) and pigeon paramyxovirus-1 (PPMV-1) were raised in rabbits to examine their diagnostic efficacy against APMV-1 and PPMV-1 infections in birds. Rabbits were divided into two groups A (immunized with APMV-1) and group B (immunized with PPMV-1). An antibody titer of 1:1024 group A against APMV-1 antigen and group B against PPMV-1 antigen were found 1:1024. While final titer of group A was found 1:256 against PPMV-1 and for group B, 1:512 against APMV-1. This study suggests that it is possible to diagnose Newcastle disease and its type by the use of these polyclonal antibodies from the field out breaks. However, the subjected serum must be examined with both antiseras. This will only suggest the type of infection. By the use of these polyclonal antibodies screening at large scale can be done and samples can be selected for further diagnosis using advanced techniques. This will in turn save time and expensive foreign exchange., Polyclonal antibodies against avian paramyxovirus-1 (APMV-1) and pigeon paramyxovirus-1 (PPMV-1) were raised in rabbits to examine their diagnostic efficacy against APMV-1 and PPMV-1 infections in birds. Rabbits were divided into two groups A (immunized with APMV-1) and group B (immunized with PPMV-1). An antibody titer of 1:1024 group A against APMV-1 antigen and group B against PPMV-1 antigen were found 1:1024. While final titer of group A was found 1:256 against PPMV-1 and for group B, 1:512 against APMV-1. This study suggests that it is possible to diagnose Newcastle disease and its type by the use of these polyclonal antibodies from the field out breaks. However, the subjected serum must be examined with both antiseras. This will only suggest the type of infection. By the use of these polyclonal antibodies screening at large scale can be done and samples can be selected for further diagnosis using advanced techniques. This will in turn save time and expensive foreign exchange., |
