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Abstract : |
Soybean (Glycine max L.) cell suspension cultures were exposed to excess copper, in liquid MS-medium. Viability, growth rate and phytochelatins concentration were monitored in treated and untreated cultures. The viability of Cu-treated cultures was reduced and more affected with the time of Cu-exposure, while the O2-uptake was slightly raised in treated cultures and decreased with the time of Cu-exposure. The growth rate was more affected at 10 and 15 days of treatment and reduced by about 76 and 83% below that of the control culture, respectively. The production of PCs in treated cultures markedly increased with the time of Cu-exposure. The glutathione depletion is coincident with PCs production. A marked increase in the level of GSSG in treated and untreated cultures with the time of Cu-exposure. The accumulation of Cu in treated cultures was raised as the time of exposure increased. The accumulation of copper is coincident with PCs levels. Phytochelatins detection seem to be an important biomarker technique in systems contamined with heavy metals. , Soybean (Glycine max L.) cell suspension cultures were exposed to excess copper, in liquid MS-medium. Viability, growth rate and phytochelatins concentration were monitored in treated and untreated cultures. The viability of Cu-treated cultures was reduced and more affected with the time of Cu-exposure, while the O2-uptake was slightly raised in treated cultures and decreased with the time of Cu-exposure. The growth rate was more affected at 10 and 15 days of treatment and reduced by about 76 and 83% below that of the control culture, respectively. The production of PCs in treated cultures markedly increased with the time of Cu-exposure. The glutathione depletion is coincident with PCs production. A marked increase in the level of GSSG in treated and untreated cultures with the time of Cu-exposure. The accumulation of Cu in treated cultures was raised as the time of exposure increased. The accumulation of copper is coincident with PCs levels. Phytochelatins detection seem to be an important biomarker technique in systems contamined with heavy metals., |
