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Abstract : |
NOT AVAILABLE, Soybean Mosaic potyvirus (SMV) is highly prevalent in major soybean growing areas of NWFP, infected plants exhibited predominantly mosaic and mottling symptoms on leaves and stunted growth due to shortened petioles and internodes. Incidence of the virus ranged from mild to severe infection between 8-72% in areas surveyed. ELISA test further confirmed the incidence of the virus from 7 to 64% in major soybean growing area of the province, during summer 1997-98. Twelve soybean mosaic virus isolates were collected from soybean crop growing in different areas on the basis of characteristics and distinguishable mosaic and mottling symptoms. Isolates, SMV-S1, S2, S3 from Swat and SMV-P1, P2, P3 from Peshawar were further selected on the basis of severity of symptoms and characterized. SMV-S1, S2, S3 produced severe mosaic on mechanically inoculated soybean (Glycine max) cv. Weber-84, leaf chlorosis and chlorotic local lesion on phaseolus valgaris and P.luntas, and local lesion on Chenopodium amaranticolor and C.quinoa SMV-P1, P2 and P3 isolates differed from Swat isolates by producing mild mosaic on soybean cv. Weber-84 isolate SMV-S1 produced severe mosaic, followed by severe mottling, necrosis and occasionally death of plants Weber-84 inoculated with serially diluted sap from infected soybean plants while SMV-P1 caused mild to moderate mosaic symptoms. The dilution end points of both isolates was 1/512 mean A405nm ELISA values of SMV-S1, S2 and S3 was 1.919 and of SMV-P1, P2 and P3 was 1.377, respectively, after overnight incubation of antigen with substrate solution at 4 أƒâ€ڑأ‚آ°C. Among these isolates, SMV-S1 showed maximum A405nm values (1.987) followed by SMV-P1 (1.477), respectively., |
