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Abstract : |
A total of 120 patients with positive cultures were tested with eight commonly used anti-tuberculosis drugs i.e. Isoniazid (H), streptomycin (S), Para aminosalicylic acid (PAS), Ethionamide (ETH), Ethambutol (E), Thiacitazon (THI), Rifampicin (R) and Pyrazinamide (Z). Standard proportion method as recommended by CDC Atlanta, Georgia (USA) using L-J medium was adopted. The isolates of M. tuberculosis were identified by their acid-fast character on Z-N staining, clonial growth on L-J medium, niacin accumulation, nitrate reduction, positive catalase test and amplification of a 541-bp fragment from IS986 gene by Polymerase Chain Reaction (PCR). Sixty one (50.83%) of the isolates were resistant to one or more anti-tuberculosis drugs. Of these resistant patients, 10 (16.39%) were resistant to only one anti tuberculosis drug while 51 (83.6%) were resistant to more than one drug. As regards resistance to individual drugs, 33 (27.5%) of the isolates were resistant to H, 22 (18.33%) to S, 42 (35%) to PAS, 21 (17.5%) to ETH, 20 (16.67%) to E, 8 (6.67%) to THI, 24 (20.0%) to R and 34 (28.34%) to Z. These findings showed a growing concern of increasing prevalence of drug resistant M. tuberculosis. , A total of 120 patients with positive cultures were tested with eight commonly used anti-tuberculosis drugs i.e. Isoniazid (H), streptomycin (S), Para aminosalicylic acid (PAS), Ethionamide (ETH), Ethambutol (E), Thiacitazon (THI), Rifampicin (R) and Pyrazinamide (Z). Standard proportion method as recommended by CDC Atlanta, Georgia (USA) using L-J medium was adopted. The isolates of M. tuberculosis were identified by their acid-fast character on Z-N staining, clonial growth on L-J medium, niacin accumulation, nitrate reduction, positive catalase test and amplification of a 541-bp fragment from IS986 gene by Polymerase Chain Reaction (PCR). Sixty one (50.83%) of the isolates were resistant to one or more anti-tuberculosis drugs. Of these resistant patients, 10 (16.39%) were resistant to only one anti tuberculosis drug while 51 (83.6%) were resistant to more than one drug. As regards resistance to individual drugs, 33 (27.5%) of the isolates were resistant to H, 22 (18.33%) to S, 42 (35%) to PAS, 21 (17.5%) to ETH, 20 (16.67%) to E, 8 (6.67%) to THI, 24 (20.0%) to R and 34 (28.34%) to Z. These findings showed a growing concern of increasing prevalence of drug resistant M. tuberculosis., |
