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Abstract : |
Cell free extracts of Fusarium moniliforme grown on L-serine as the sole source of nitrogen contained L-serine dehydratase which catalyzes the cleavage of L-serine into equimolar amounts of pyruvate and ammonia. L-serine dehydratase produced during the logarithmic phase. Maximal growth and enzyme production were obtained after 5 days incubation. Of the tested metal salts, FeSO4 was the best inducer for L-serine dehydratase synthesis by Fusarium moniliforme. The optimum pH for growth and L-serine dehydratase formation was 5. L-serine dehydratase was induce by a great variety of nitrogen sources, but L-serine and L-threonine were the best inducer. L-Serine concentration of 2.4 g L-1 was the optimal for L-serine dehydratase synthesis. The effect of different carbon sources on growth and enzyme formation was investigated., Cell free extracts of Fusarium moniliforme grown on L-serine as the sole source of nitrogen contained L-serine dehydratase which catalyzes the cleavage of L-serine into equimolar amounts of pyruvate and ammonia. L-serine dehydratase produced during the logarithmic phase. Maximal growth and enzyme production were obtained after 5 days incubation. Of the tested metal salts, FeSO4 was the best inducer for L-serine dehydratase synthesis by Fusarium moniliforme. The optimum pH for growth and L-serine dehydratase formation was 5. L-serine dehydratase was induce by a great variety of nitrogen sources, but L-serine and L-threonine were the best inducer. L-Serine concentration of 2.4 g L-1 was the optimal for L-serine dehydratase synthesis. The effect of different carbon sources on growth and enzyme formation was investigated., |
