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Abstract : |
In this study, a pair of degenerate PCR primer was designed base on the known LMW-GS genes, with which 4 distinct sequences were obtained from a weak gluten variety of wheat, Chuannong 16, designated as LMWCN16-1, LMWCN16-2, LMWCN16-3 and LMWCN16-4, respectively. The total lengths of the 4 genes were 902, 915, 909 and 927 bp, respectively. All these genes were predicted to be located at Glu-D3 locus and amount of DNA variations were found, including 97 nucleotide substitutions and several insertions and deletions (indels). Sequence analysis indicated that the pairwise identities between the 4 genes were from 81.9 to 95.6%. LMWCN16-2 and LMWCN16-3 were more close related, while LMWCN16-4 was distinguished from LMWCN16-2 and LMWCN16-3 by a longer repetitive domain and the different position of the seventh cysteines involved in inter-molecular disulphide bond. These differences might result in different effects on the formation of the glutenin polymer and consequently the wheat quality. LMWCN16-1 is a putative pseudogene due to the five inframe stop codons and the frame shifting mutation in its C-terminal domain. The function loss of LMWCN16-1 might weaken the proportion of Glu-D3 in the total LMW-GS and bring negative influence on wheat quality., |
