Home

Factors Affecting the Callus Induction and GUS Transient Expression in Indica Rice Pei`ai64s


Author(s) : Xue Qingzhong Xianyin Zhang Haifeng Qian, 
Publisher : N/A
Publication Date : 2004
ISSN : N/A
Abstract : In the current study, the effect of different concentrations of 6-benzyladenine, the effect of light on callus induction, browning and GUS transient expression in Indica rice Peiai64s was investigated. The results indicated that embryos callus from seeds and GUS transient expression is favored by the addition of concentration of 6-benzyladenine to cultured medium and culturing under light condition. High 6-benzyladenine concentration(0.5, 1 mg L-1 )reduces the frequency of callus, GUS transient expression and enhances browning ratio. Light was also observed as browning for promoting factor, In the current study, the effect of different concentrations of 6-benzyladenine, the effect of light on callus induction, browning and GUS transient expression in Indica rice Pei?ai64s was investigated. The results indicated that embryos callus from seeds and GUS transient expression is favored by the addition of concentration of 6-benzyladenine to cultured medium and culturing under light condition. High 6-benzyladenine concentration(0.5, 1 mg L-1 )reduces the frequency of callus, GUS transient expression and enhances browning ratio. Light was also observed as browning for promoting factor,