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Abstract : |
NOT AVAILABLE, Tissue culture of Basmati rice was done to study the effect of growth hormones on the formation of callus. The varieties used were 'Basmati 370' Basmati 385'and 'Basmati 198'. The medium used was Murashige and Skoog (MS-medium). Different plant portions like seeds, roots and shoots were used as explants and were culture on Ms-medium supplemented with different combinations of plant growth hormones. The hormones used were 2,4 Dichlorophexy acetic acid (2,4-D) and kinetin-Basmati-370 was the best for callus induction. Best callus induction and vigorous growth was observed from seeds cultured on media containing 0.5 mg/l kinetin 2,4-D. The response of roots and shoot expalnts to callogenesis was little as compared to seeds explant. The mature seeds of Basmati rice are the best source of callus., |