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Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7


Author(s) : Lihan Samuel Endang Purwati Karim Mohamed Ismail Abdul Gulam Rusul Son Radu Ling Ooi Wai, 
Publisher : N/A
Publication Date : 2000
ISSN : N/A
Abstract : A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.,