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Abstract : |
Microshoots were cultured on MS medium supplemented with different combinations of auxin, sucrose and pH level for in vitro rooting of callus induced sugarcane (Saccharum officinarum) variety namely Isd 31. Among the types and concentrations of auxin used the best rooting in all the microshoots was obtained with half strength (major salts) MS basal medium supplemented with NAA at 5.0 mg lأƒآ¢أ‚€أ‚آ¾1, sucrose at 50 g lأƒآ¢أ‚€أ‚آ¾1 and pH level 5.7 were the optimum conditions for rooting when microshoots were incubated 25+ 2أƒâ€ڑأ‚آ°C under 16 h photo period regime. The regenerated plantlets were successfully transferred to soil and the percentage of survivability under ex vitro conditions was 75., |
