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Abstract : |
In the present research a novel thermophilic fungi-Mycotypha sp. strain No. AKM1801 was used to evaluate its efficiency for endo-pectinase production from coffee pulp, a waste produced during coffee processing. The culture was cultivated on coffee pulp through submerged fermentation at 45?2?C in aerated and stationary conditions. Mycotypha showed the maximum endo-pectinase activity of 5.4 U mL-1 at 96 h at pH 5.4 and 4.9 U mL-1 at 168 h at pH 5.6 in aerated and stationary conditions, respectively. The pectin content was reduced up to 85% in both conditions. Maximum decolorization was achieved in stationary condition. The present investigation was conducted to study the physiological behavior of Mycotypha during dimorphic structure i.e., yeast phase in aerated and mycelial phase in stationary for endo-pectinase production. This could be highly beneficial for the production of microbial enzymes from coffee pulp in food and beverage based biotechnological industries., |