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Abstract : |
Vigorously growing root tips, basal discs and leaf discs of three garlic strains (G-101, G- 102, G-103) were used as explants. The explants were collected from cloves germinated in a basal medium. Different combinations and concentrations of growth regulators like 2, 4-D and BAP were used in MS medium to observe the callus induction, proliferation, organogenesis and to produce regenerate plantlets under in vitro condition. The highest callusing was recorded at the highest concentration of 2, 4-D (2.0 mg l-1) + BAP (1.0 mg l-1) in MS medium for G-103 (80%). The other two strains (G-101, G-102) showed 70 and 75% callusing ability respectively. Highest performance in the proliferation of calli were found at MS+2,4-D (2.0 mg l-1) +BAP (0.5 mg l-1) in G-103 (90%). Maximum number of regenerated plantlets were obtained at MS + NAA (2.0 mg l-1) + BAP (1.0 mg l-1) in all the strains (35%). Plantlets thus, obtained were successfully transferred to the potted soil and subsequently to the field. The survival rate was 40-60% in all the cases., |
