|
Abstract : |
High production of cell biomass and somatic embryos could be established in the suspension cultures of Cyperus aromaticus. The callus derived from root pieces was used for preparation of the cell suspension cultures. The cells pretreated with 2,4-Dichlorophenoxyacetic acid (2,4-D), α-Naphthaleneacetic Acid (NAA) and picloram in the suspension cultures produced somatic embryos after one week of transferring onto Murashige and Skoog (MS) solid medium without Plant Growth Regulators (PGR). With a small amount of cell inoculum (0.12 g) cultured in liquid MS medium supplemented with 2.0 mg L-1 NAA for three weeks followed by one week culture on solid MS basic medium produced an average of 10.5 g fresh embryogenic cell biomass of C. aromaticus. The embryogenic cells differentiated to produce primodia leaves after transferred to MS medium supplemented with 1.0 mg L-1 NAA. , High production of cell biomass and somatic embryos could be established in the suspension cultures of Cyperus aromaticus. The callus derived from root pieces was used for preparation of the cell suspension cultures. The cells pretreated with 2,4-Dichlorophenoxyacetic acid (2,4-D), أƒإ½أ‚آ±-Naphthaleneacetic Acid (NAA) and picloram in the suspension cultures produced somatic embryos after one week of transferring onto Murashige and Skoog (MS) solid medium without Plant Growth Regulators (PGR). With a small amount of cell inoculum (0.12 g) cultured in liquid MS medium supplemented with 2.0 mg L-1 NAA for three weeks followed by one week culture on solid MS basic medium produced an average of 10.5 g fresh embryogenic cell biomass of C. aromaticus. The embryogenic cells differentiated to produce primodia leaves after transferred to MS medium supplemented with 1.0 mg L-1 NAA., |
