Dealing with different methods for Kluyveromyces lactis &bgr;-galactosidase purification
| Author(s) : | MI Gonzãlez Siso Cerdãn E Becerra M, |
| Publisher : | N/A |
| Publication Date : | 1998 |
| ISSN : | N/A |
| Abstract : | Several micro-scale chromatography-based procedures for purification of the &bgr;-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme., |