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Abstract : |
During March and April 2007, 140 native cows with the mean age of more than one year were selected randomly and their age and sex were registered in the related papers. Primarily, a thin layer smear was prepared from their ear sublime vein blood and was fixed with methanol and then it stained with Gimsa dye. Also, 9 mL blood from the jagular vein of the same cow was taken and collected in autoclaved tubes (containing 1 mL of 3.2% buffered citrate solution) conversely for extracting their DNA for PCR. Two primers were used: N516/N517 belonged to a huge gene (30 kDa) which is responsible for coding the surface antigen of Theileria annulata merozoite. By detection of 140 prepared blood smear with light microscope and lens 100, piroplasmic forms of Theileria annulata were seen in 12 smear (8.57%). By PCR method, 56 DNA of Theileria annulata was separatedأƒâ€ڑأ‚آ fromأƒâ€ڑأ‚آ 140أƒâ€ڑأ‚آ samplesأƒâ€ڑأ‚آ (40%). Statistical comparison of PCR and smear methods in diagnosing of Theileria annulata carriers can explain a significant difference between these two methods (p<0.05). The results of this study shows that sensitivity and accuracy of PCR method in diagnosing of Theileria annulata carriers is more than common method of smear preparation and can be used in epidemiological studies for the sake of controlling, prevention and determining of immunological condition of cows in region., |
