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Abstract : |
A protocol has been developed for callus induction, regeneration and hygromycin selection of rice (Oryza sativa L. cv. Super Basmati). Mature seeds of rice were used as starting material for callus induction and cultured on Murashige & Skoog medium (MS) and N6 medium having different levels and combinations of 2,4-Dicholro Phenoxy Acetic Acid (2,4-D). 2,4-D @ 2 mg/l gave good response to callus induction. For regeneration, calli were cultured on MS medium having different concentrations of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP). Medium containing NAA @ 1mg/l with BAP 5 mg/l showed best results with regeneration frequency of 45.3%. Hygromycin at the concentration of 50 mg/l proved to be lethal for secutellum-darived calli of Super Basmati., |
