|
Abstract : |
Turpentine oils, gum oleoresins and rosins from Pinus merkusii Jungh et de Vrises were collected on the island of Sumatra and Java for investigation of their chemical composition. Gum oleoresins were separated into neutral and acidic fractions with an aqueous 4% sodium hydroxide solution. The fractions containing turpentine oils and rosins were analyzed by Gas Chromatography (GC) and Gas Chromatograph Mass Spectrometry (GC-MS), respectively. The neutral fractions of gum oleoresins and turpentine oils mainly consisted of α-pinene, Δ-3-carene and β-pinene. A difference in chemical composition with locality was recognized for the turpentine oils and neutral fractions of gum oleoresins. While examining the chemical composition of the acidic fractions of gum oleoresins and rosins, we found that relative retention time could not be used to identify each resin acid. Based on mass spectral comparison, the major constituents of the acidic fractions and rosins were identified as sandaracopimaric acid, isopimaric acid, palustric acid, dehydroabietic acid, abietic acid, neoabietic acid and merkusic acid. The major component of the acidic fractions was palustric acid, while that of both rosin 1 and 2 was abietic acid. The chemical composition of the acidic fractions of gum oleoresins and rosins differed with locality. Using TC-1 and TC-5 columns, levopimaric acid could not be separated from rosins or acidic fractions of gum oleoresins of Indonesian Pinus merkusii. This is the first report that neither fraction contains levopimaric acid., Turpentine oils, gum oleoresins and rosins from Pinus merkusii Jungh et de Vrises were collected on the island of Sumatra and Java for investigation of their chemical composition. Gum oleoresins were separated into neutral and acidic fractions with an aqueous 4% sodium hydroxide solution. The fractions containing turpentine oils and rosins were analyzed by Gas Chromatography (GC) and Gas Chromatograph Mass Spectrometry (GC-MS), respectively. The neutral fractions of gum oleoresins and turpentine oils mainly consisted of أƒإ½أ‚آ±-pinene, أƒإ½أ‚â€‌-3-carene and أƒإ½أ‚آ²-pinene. A difference in chemical composition with locality was recognized for the turpentine oils and neutral fractions of gum oleoresins. While examining the chemical composition of the acidic fractions of gum oleoresins and rosins, we found that relative retention time could not be used to identify each resin acid. Based on mass spectral comparison, the major constituents of the acidic fractions and rosins were identified as sandaracopimaric acid, isopimaric acid, palustric acid, dehydroabietic acid, abietic acid, neoabietic acid and merkusic acid. The major component of the acidic fractions was palustric acid, while that of both rosin 1 and 2 was abietic acid. The chemical composition of the acidic fractions of gum oleoresins and rosins differed with locality. Using TC-1 and TC-5 columns, levopimaric acid could not be separated from rosins or acidic fractions of gum oleoresins of Indonesian Pinus merkusii. This is the first report that neither fraction contains levopimaric acid., |
