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Abstract : |
Growth rate and branching of D. uninucleata were correlated to the exocellular concentration of calcium in culture medium. Calcium concentrations of 1-10 mM stimulated germ tube formation and reduced the lag phase. Mycelia grew in media amended with 1-0 mM Ca2+ were highly branched and the number of cells/hypha together with the number of cells/branch significantly increased. Gametangial formation have been induced by 10-40 mM Ca2+ and the highest percent of ascus formation with the longest asci was achieved in the presence of 10mM calcium. Increasing Ca2+ ions concentration above 40 mM had no further effect on growth aspects yet concentrations higher than 160 mM were toxic and partially inhibited growth, branching and reproduction. , Growth rate and branching of D. uninucleata were correlated to the exocellular concentration of calcium in culture medium. Calcium concentrations of 1-10 mM stimulated germ tube formation and reduced the lag phase. Mycelia grew in media amended with 1-0 mM Ca2+ were highly branched and the number of cells/hypha together with the number of cells/branch significantly increased. Gametangial formation have been induced by 10-40 mM Ca2+ and the highest percent of ascus formation with the longest asci was achieved in the presence of 10mM calcium. Increasing Ca2+ ions concentration above 40 mM had no further effect on growth aspects yet concentrations higher than 160 mM were toxic and partially inhibited growth, branching and reproduction., |
