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Abstract : |
The Rhizobium isolates collected from various ecological conditions of Azad Jammu and Kashmir were characterized using conventional and molecular techniques. The conventional techniques like the antibiotic resistance and in vitro and in vivo nodulation tests proved that there exists large diversity among the isolates. The isolates showed great potential for BNF under natural conditions, however, their potential could only be exploited if their genetic and biochemical markers are identified. The molecular techniques like plasmid profiles and the cellular protein profiles were investigated to identify some marker genes and their protein products for practical BNF and for the manipulation of the DNA based markers for better activity of the bacteria in plant nodules. The isolates differed in nodulation test also showed variability in their plasmid and protein profiles. The relationship between plasmid and protein profile depicts the presence of the variable genes on plasmids, which produce their influence in the form of protein products. These proteins may have their role against the environmental stresses in which the bacteria are adopted. The status of the genes once confirmed would be of great importance because plasmid are easy to handle, easy to manipulate and easy to transfer from one strain to another strain which may have otherwise desirability in BNF programme, |
