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Abstract : |
Karyotypic analysis was performed for 11 genotypes of chickpea (Cicer arietinum L.) in order to determine their potential genetic relationships. Pretreatment of root tips in 8-hydroxyquinoline, followed by fixation in Lewitsky solution and hydrolysis in 1N NaOH at 60°C, then staining in aceto-iron-hematoxylin for 15-20 h, confirmed a somatic chromosome number of 2n = 16 in all genotypes. This karyotype consisted of: a pair with the longest satellited submetacentric chromosome, a pair with the shortest metacentric chromosome and six pairs of metacentric to submetacentric chromosomes. Intraspecific variabilities were observed for haploid chromosome length, primary constriction position and karyotypic formula. But all of the studied genotypes showed the same karyotype asymmetry, 1B. Haploid genome length ranged from 24.88 μm±0.50 in G6 (Bieanij cv) to 18.48±0.60 μm in G10 (Flip93-93c cv). Cluster analysis classified the 11 genotypes in three groups, having different sizes of chromosomes and arms., Karyotypic analysis was performed for 11 genotypes of chickpea (Cicer arietinum L.) in order to determine their potential genetic relationships. Pretreatment of root tips in 8-hydroxyquinoline, followed by fixation in Lewitsky solution and hydrolysis in 1N NaOH at 60أƒâ€ڑأ‚آ°C, then staining in aceto-iron-hematoxylin for 15-20 h, confirmed a somatic chromosome number of 2n = 16 in all genotypes. This karyotype consisted of: a pair with the longest satellited submetacentric chromosome, a pair with the shortest metacentric chromosome and six pairs of metacentric to submetacentric chromosomes. Intraspecific variabilities were observed for haploid chromosome length, primary constriction position and karyotypic formula. But all of the studied genotypes showed the same karyotype asymmetry, 1B. Haploid genome length ranged from 24.88 أƒإ½أ‚آ¼mأƒâ€ڑأ‚آ±0.50 in G6 (Bieanij cv) to 18.48أƒâ€ڑأ‚آ±0.60 أƒإ½أ‚آ¼m in G10 (Flip93-93c cv). Cluster analysis classified the 11 genotypes in three groups, having different sizes of chromosomes and arms., |
